694-701 Agrobacterium tumefaciens is a soil phytopathogen that naturally infects plant wound sites and causes crown gall disease via delivery of transferred (T)-DNA from bacterial cells into host plant cells through a bacterial type IV secretion system (T4SS). Products of the other VirB genes are used to transfer the subunits across the The T-DNA must be cut out of the circular plasmid. Plant Cell Rep 5:81–84 CrossRef Google Scholar. Particle-bombardment-mediated co-transformation of elite Chinese rice cultivars with genes conferring resistance to bacterial blight and sap-sucking insect pests. The integration of transferred DNA (T-DNA) and the formation of complex insertions in the genome of transgenic plants during Agrobacterium -mediated transformation are still poorly understood.
2014 Jun 18;10:19. doi: 10.1186/1746-4811-10-19. However, only in the past two decades has the ability of Agrobacterium to transfer DNA to plant cells been harnessed for the purposes of plant genetic engineering. Agrobacterium-transformed of rice plants expression cryIA(b) and cryIA(c) genes are highly toxic to striped stem borer and yellow stem borer. Stz-3-03) Supported by the Ministry of Finance.National Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200032, ChinaNational Laboratory of Plant Genetic Engineering and Protein Engineering, College of Life Science, Peking University, Beijing, 100871, ChinaYou can also search for this author in Rapid isolation of high molecular weight plant DNA. One pair of specific primers were used to detect the npt II gene: forward primer (P1) 5′-3.
DNA was extracted from leaf tissue of 45 TSouthern blot analysis of transgenic rice plants P: Positive control (2.0kb XhoI fragment from pExTAfter transplanting to soil, all transgenic rice plants grew well and produced normal seeds (Our long-term goals are to improve the quality of rice by using an approach in which multiple disease- and insect-resistant traits can be introduced into the same lines In conclusion, we have obtained over 970 transgenic rice plants by Agrobacterium-mediated transformation procedure. Chyi YS, Phillips GC (1987) High efficiency Agrobacterium-mediated transformation of Lycopersicon based on conditions favorable for regeneration. Stanton B. Gelvin, Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907-1392, Agrobacterium tumefaciens is a plant pathogenic bacterium that contains a plasmid (the tumour-inducing or Ti plasmid), part of which (the T-DNA) integrates into the host plant chromosomes (Box 1.7); it has been termed “nature’s genetic engineer”.For plant transformation, the tumour-inducing genes are removed to make a “disarmed” plasmid. Cell suspension and protoplast culture in rice. 15 mutations, including nucleotide deletions and substitutions, were identified in 14 lines from 90 independent transgenic plants (Figure 1 b), … Modern plant biotechnology revolves …
This technology is also useful for identifying and evaluating agriculturally useful traits (genes) as well as for their introduction into commercially valuable crops. This information has been applied to develop methods that result in the formation of gall-free, genetically transformed plants. Feeding assays with T1 plants from 10 independent transgenic lines confirmed that the transgenic plants were highly toxic to LF and SSB larvae.Tang KX, Porntip T, Xu YN, Sun XF, Gatehouse JA, Ronald PC, Qi HX, Lu XG, Christou P, Kohli A . SUMMARY Agrobacterium tumefaciens and related Agrobacterium species have been known as plant pathogens since the beginning of the 20th century. Media used for tissue culture and transformation are listed in The calli were soaked in bacterial suspension for 20 min, blotted dry with sterile filter paper to remove excess bacteria. Huang JQ, Wei ZM, An HL, Xu SP, Zhang B .