Meletiadis, J., Pournaras, S., Roilides, E. & Walsh, T. J. You can also search for this author in Quinlan, A. R. & Hall, I. M. BEDTools: a flexible suite of utilities for comparing genomic features. A. et al.
Hernandez, D., Francois, P., Farinelli, L., Osteras, M. & Schrenzel, J. To generate isogenic strains with or without pCD-METRO, we introduced a shuttle module in the unique Overall, our results show that acquisition of pCD-METRO is sufficient to raise the MIC of Read depth of pCD-METRO in our WGS data indicates an estimated copy number of 100–200, in stark contrast with the pCD6 replicon commonly used in shuttle vectors for Next, we compared the relative copy number of the plasmids in overnight cultures by quantitative PCR (qPCR)A difference between the read-depth estimate and the qPCR can be explained by technical bias or differences in strain background.
Otherwise, data were analyzed using non-parametric ANOVA with post-hoc Dunn’s multiple comparison test.No datasets were generated or analysed during the current study.Lawson, P. A., Citron, D. M., Tyrrell, K. L. & Finegold, S. M. Reclassification of CDC. Gibson, D. G. et al. Then 10 µL of bacterial inoculum was added to each well and incubated for 48 h under anaerobic conditions at 37 °C. Carver, T., Thomson, N., Bleasby, A., Berriman, M. & Parkhill, J. DNAPlotter: circular and linear interactive genome visualization. Clostridium difficile infection in Europe: a hospital-based survey.
Coordinators, N. R. Database resources of the National Center for Biotechnology Information. Solitary Spore is a major transmissive agent in CDI as vegetative cells cannot torelate aerobic environmentTicagrelor, formerly known as AZD6140, is a synthetic compound mimicking ATP formulated for an oral administration. A., Sussman, D. A., Fifadara, N. & Barkin, J. S. Clostridium difficile infection and patient-specific antimicrobial resistance testing reveals a high metronidazole resistance rate.
van Eijk, E. et al. 2019 update of the WSES guidelines for management of Clostridioides (Clostridium) difficile infection in surgical patients. Analyzed the data: M.P. Defining fractional inhibitory concentration index cutoffs for additive interactions based on self-drug additive combinations, Monte Carlo simulation analysis, and Leuzzi, R., Adamo, R. & Scarselli, M. Vaccines against Serpi, M., Ferrari, V. & Pertusati, F. Nucleoside derived antibiotics to fight microbial drug resistance: new utilities for an established class of drugs? You are using a browser version with limited support for CSS. To obtain All Annotation was performed with an in-house pipeline as described beforeExtrachromosomal elements were predicted as described beforeSNP typing was performed after selecting the best reference assembly. Antimicrobial susceptibility and mechanisms of resistance of Greek Clostridium difficile clinical isolates. McDonald, L. C. et al.
A. et al. Performed the experiments: M.P. Debast, S. B., Bauer, M. P. & Kuijper, E. J. European Society of Clinical Microbiology and Infectious Diseases: update of the treatment guidance document for Clostridium difficile infection. Next, we sought to unambiguously demonstrate that acquisition of pCD-METRO, and not any secondary events, lead to metronidazole resistance.
and JavaScript.At present, drug repurposing, or drug repositioning, has gained tremendous attention as an alternative approach for drug development as it shortens the process of drug discovery by nearly a half, as the information on drugs toxicity, pharmacokinetics, and dosages is readily retrievableWe first tested the antimicrobial activity of ticagrelor against different ribotypes of After the antimicrobial activity of ticagrelor was confirmed, we investigated the killing kinetics of ticagrelor with Ticagrelor exhibits a bactericidal activity against We further investigated the effect of ticagrelor on biofilm formation at the sub-MICs and MIC level with The amount of proteins and DNA leaked from the bacterial cells were measured by Bradford protein assay and NanoDrop spectrophotometer, respectively. Spigaglia, P. Recent advances in the understanding of antibiotic resistance in Clostridium difficile infection. Pelaez, T. et al.
Bauer, M. P. et al.
Smits, W. K., Lyras, D., Lacy, D. B., Wilcox, M. H. & Kuijper, E. J. Clostridium difficile infection.
Contributed patient samples and metadata: E.M.T., E.J.K. & Sullivan, N. E. Nitroimidazoles: in vitro activity and efficacy in anaerobic infections. The corresponding author had full access to all the data in the study and had final responsibility for the decision to submit for publication.W.K.S. Clostridium difficile, a Gram-positive, spore-forming anaerobic bacterium, is the leading cause of infectious diarrhea among patients in hospitals worldwide, causing C. difficile infection (CDI).
Smits, W. K., Weese, J. S., Roberts, A. P., Harmanus, C. & Hornung, B.